|
Primer name |
Primer sequence |
Length |
Exp.
annealing |
Allele size range (bp) |
| ssrVrZAG7[F] | gtggtagtgggtgtgaacggagtgg | 25 | 50 | 106-158 |
| ssrVrZAG7[R] | aacagcatgacatccacctcaacgg | 25 | ||
| ssrVrZAG12 [F] | ctgcaaataaatattaaaaaattcg | 25 | 50 | 140-172 |
| ssrVrZAG12 [R] | aaatcctcggtctctagccaaaagg | 25 | ||
| ssrVrZAG14 [F] | atcaaaggcctcctttattgccatc | 25 | 50 | 137-162 |
| ssrVrZAG14 [R] | tagtaccaactaccaacaaccaaag | 25 | ||
| ssrVrZAG15 [F] | ggattttggctgtagttttgtgaag | 25 | 50 | 163-193 |
| ssrVrZAG15 [R] | atctcaagctgggctgtattacaat | 25 | ||
| ssrVrZAG21 [F] | tcattcactcactgcattcatcggc | 25 | 50 | 190-214 |
| ssrVrZAG21 [R] | ggggctactccaaagtcagttcttg | 25 | ||
| ssrVrZAG25 [F] | ctccacttcacatcacatggcatgc | 25 | 50 | 225-245 |
| ssrVrZAG25 [R] | cggccaacatttactcatctctccc | 25 | ||
| ssrVrZAG26 [F] | acatcgtagtatataaatagcgacg | 25 | 50 | No amplification in V. vinifera |
| ssrVrZAG26 [R] | tattctagccctactctgcactgtc | 25 | ||
| ssrVrZAG29 [F] | ataaccaggacaagttattcaagcc | 25 | 50 | 112-122 |
| ssrVrZAG29 [R] | acccaattgaccatcttttatgctg | 25 | ||
| ssrVrZAG30 [F] | tggtaaacctagaaaaattcatcaa | 25 | 50 | 143-151 |
| ssrVrZAG30 [R] | catatgaccatgtcactaaattaat | 25 | ||
| ssrVrZAG47 [F] | ggtctgaatacatccgtaagtatat | 25 | 50 | 149-172 |
| ssrVrZAG47 [R] | acggtgtgctctcattgtcattgac | 25 | ||
| ssrVrZAG62 [F] | ggtgaaatgggcaccgaacacacgc | 25 | 50 | 185-203 |
| ssrVrZAG62 [R] | ccatgtctctcctcagcttctcagc | 25 | ||
| ssrVrZAG64 [F] | tatgaaagaaacccaacgcggcacg | 25 | 58 | 137-197 |
| ssrVrZAG64 [R] | tgcaatgtggtcagcctttgatggg | 25 | ||
| ssrVrZAG67 [F] | acctggcccgactcctcttgtatgc | 25 | 45 | 126-159 |
| ssrVrZAG67 [R] | tcctgccggcgataaccaagctatg | 25 | ||
| ssrVrZAG79 [F] | agattgtggaggagggaacaaaccg | 25 | 50 | 236-260 |
| ssrVrZAG79 [R] | tgcccccattttcaaactcccttcc | 25 | ||
| ssrVrZAG82 [F] | ttctctcccttgctcctctctgtgg | 25 | 50 | No amplification in V. vinifera |
| ssrVrZAG82 [R] | tgccatgaccttgaaggaaatctag | 25 | ||
| ssrVrZAG83 [F] | ggcggaggcggtagatgagagggcg | 25 | 50 | 186-200 |
| ssrVrZAG83 [R] | acgcaacggctagtaaatacaacgg | 25 | ||
| ssrVrZAG93 [F] | tatggagggaccgaggtgggctagg | 25 | 50 | 188-208 |
| ssrVrZAG93 [R] | gcactcttcgacgttaaacaaagcc | 25 | ||
| ssrVrZAG112 [F] | cgtttaaagccagctgaatcttggg | 25 | 50 | 229-261 |
| ssrVrZAG112 [R] | tggctccatactgcttcacgtaggc | 25 |
Nuclear SSR
primers of the Division of Horticulture CSIRO, Adelaide, Australia
Thomas MR, Scott NS (1993) Microsatellite repeats in grapevine
reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs).
Theor Appl Genet, 86:985-990.
Thomas MR, Scott NS, Botta R, Kijas JMH (1998) Sequence-tagged
site markers in grapevine and citrus. Journal of the Japanese Society
for Horticultural. Science 67: 1189- 1192.
|
Primer
name
|
Primer
sequence
|
Length
in bases
|
Annealing
temperature
|
Allele
size range (bp)
|
| VVS1 [F] | acaattggaaaccgcgtggag | 21 | 60.5 | 160-205 |
| VVS1 [R] | cttctcaatgatatctaaaaccatg | 25 | 51 | |
| VVS2 [F] | cagcccgtaaatgtatccatc | 21 | 53.4 | 129-155 |
| VVS2 [R] | aaattcaaaattctaattcaactgg | 25 | 52.1 | |
| VVS3 [F] | tgccctatcaattagttcaccta | 23 | 55.2 | 214-222 |
| VVS3 [R] | tcgactttgatatattgatgatt | 23 | 47.6 | |
| VVS4 [F] | ccatcagtgataaaacctaatgcc | 24 | 55.6 | 167-187 |
| VVS4 [R] | cccaccttgcccttagatgtta | 22 | 56.7 | |
| VVS5 [F] | attgatttatcaaacaccttctacat | 26 | 51.5 | 90-148 |
| VVS5 [R] | tagaaagatggaaggaatggtgat | 24 | 54.7 |
Nuclear
SSR primers of the Department of Viticulture and Enology , University
of California, Davis, USA.
Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation
and characterization of new polymorphic simple sequence repeat loci
in grape (Vitis vinifera L.). Genome 39:628-633.
Bowers JE, Dangl GS, Meredith CP (1999) Development and characterization
of additional microsatellite DNA markers for grape. Am J Enol Vitic,
50(30):243-246.
|
Primer
name
|
Primer
sequence
|
Length
in bases
|
Annealing
temperature
|
Allele
size range (bp)
|
| VVMD5-F | ctagagctacgccaatccaa | 20 | 56 | 226-246 |
| VVMD5-R | tataccaaaaatcatattcctaaa | 24 | ||
| VVMD6-F | atctctaaccctaaaaccat | 20 | 52 | 194- 214 |
| VVMD6-R | ctgtgctaagacgaagaaga | 20 | ||
| VVMD7-F | agagttgcggagaacaggat | 20 | 52 | 233- 263 |
| VVMD7-R | cgaaccttcacacgcttgat | 20 | ||
| VVMD8-F | taacaaacaagaagaggaat | 20 | 54 | 141- 164 |
| VVMD8-R | agcacatccacaacataatg | 20 | ||
| VVMD14 F | catgaaaaaatcaacataaaagggc | 25 | 56 | 222-250 |
| VVMD14 R | ttgttacccaaacacttcactaatgc | 26 | ||
| VVMD17 F | tgactcgccaaaatctgacg | 20 | 56 | 212-236 |
| VVMD17 R | cacacatatcatcaccacacgg | 22 | ||
| VVMD21 F | ggttgtctatggagttgatgttgc | 24 | 56 | 243-266 |
| VVMD21 R | gcttcagtaaaaagggattgcg | 22 | ||
| VVMD24 F | gtggatgatggagtagtcacgc | 22 | 56 | 208-219 |
| VVMD24 R | gattttaggttcatgttggtgaagg | 25 | ||
| VVMD25 F | ttccgttaaagcaaaagaaaaagg | 24 | 56 | 243-275 |
| VVMD25 R | ttggatttgaaatttattgagggg | 24 | ||
| VVMD26 F | gagacgactggtgacattgagc | 22 | 56 | 249-265 |
| VVMD26 R | ccatcaccaccatttctactgc | 22 | ||
| VVMD27 F | gtaccagatctgaatacatccgtaagt | 27 | 56 | 173-194 |
| VVMD27 R | acgggtatagagcaaacggtgt | 22 | ||
| VVMD28 F | aacaattcaatgaaaagagagagagaga | 28 | 56 | 221-279 |
| VVMD28 R | tcatcaatttcgtatctctatttgctg | 27 | ||
| VVMD31 F | cagtggtttttcttaaagtttcaagg | 26 | 56 | 196-224 |
| VVMD31 R | ctctgtgaaagaggaagagacgc | 23 | ||
| VVMD32 F | tatgattttttaggggggtgagg | 23 | 56 | 239-273 |
| VVMD32 R | ggaaagatgggatgactcgc | 20 | ||
| VVMD34 F | ggtacatcagtacttgaaatggttgc | 26 | 56 | 224-248 |
| VVMD34 R | ttctccgtagaagcgtaaacagc | 23 | ||
| VVMD36 F | taaaataataatagggggacacggg | 25 | 56 | 244-315 |
| VVMD36 R | gcaactgtaaaggtaagacacagtcc | 26 |
Nuclear
SSR primers of the Centre for Plant Conservation Genetics and the
Australian Agriculture Research Institute, Southern Cross University,
Lismore, Australia.
(Australian Patent Application PQ1276, Applicant:
Australian Rural Group Ltd., Title: Vitaceae Microsatellites)
Scott KD, Eggler P, Seaton G, Rosseto EM, Ablett EM, Lee LS, Henry
RJ (2000) Analysis of SSRs derived from grape ESTs. Theor Appl
Genet 100:723-726.
|
Primer name |
Primer sequence |
Length in bases |
Annealing temperature |
Expected length of PCR products |
| scu01vv F | tttggaatccgtcacgag | 18 | 52.3 | 164 |
| scu01vv R | ggttgacctttttccgac | 18 | ||
| scu02vv F | ttgcaagaatdcggcacgagg | 21 | 55.0 | 144 |
| scu02vv R | gaattgtgcgtggtaggctca | 21 | ||
| scu03vv F | ttcggcacgaggttttag | 18 | 54.1 | 165 |
| scu03vv R | attaggcagagaagagcgg | 19 | ||
| scu04vv F | tgtcctctttccctctcccaac | 22 | 55.2 | 175 |
| scu04vv R | cagtctgtcatctgaccatgtagcc | 25 | ||
| scu05vv F | caagcagttattgaagctgcaagg | 24 | 51.2 | 174 |
| scu05vv R | tcatccatcacacaggaaacagtg | 24 | ||
| scu06vv F | cctaatgccaggaaggttgc | 20 | 49.7 | 171 |
| scu06vv R | ccctagtctctctacctatccatg | 24 | ||
| scu07vv F | ccgaagaggaatatgggtttgag | 23 | 54.9 | 203 |
| scu07vv R | cctaacttgaaacgaaaggactgc | 24 | ||
| scu08vv F | cgagacccagcatcgtttcaag | 22 | 57.7 | 180 |
| scu08vv R | gcaaaatcctccccgtacaagtc | 23 | ||
| scu09vv F | aagcagcagttattggcg | 18 | 50.7 | 122 |
| scu09vv R | cagatactgagggtttaagctc | 22 | ||
| scu10vv F | tacccccacaaccctttttccc | 22 | 55.9 | 205 |
| scu10vv R | ttctccgccacctccttttcac | 22 | ||
| scu11vv F | aattgatagtgccacgttctcgcc | 24 | 57.3 | 248 |
| scu11vv R | aacgccgacaagaatcccaagg | 22 | ||
| scu12vv F | gaattcggcacgaggaacta | 20 | 50.0 | 130 |
| scu12vv R | acagtggagaggtgaatgca | 20 | ||
| scu13vv F | aattcggcacgagtcaacacc | 21 | 53.9 | 188 |
| scu13vv R | gctttgggaaatggagtacagaag | 24 | ||
| scu14vv F | ctgcacttgaatacgagcaggtc | 23 | 53.6 | 182 |
| scu14vv R | tgttatatgatcctccccctcctc | 24 | ||
| scu15vv F | gcctatgtgccagaccaaaaac | 22 | 53.5 | 195 |
| scu15vv R | ttggaagtagccagcccaaccttc | 24 | ||
| scu16vv F | caaagacaaagaagccaccgac | 22 | 54.4 | 170 |
| scu16vv R | accctctaaagcacacacaggaac | 24 |